Introduce heterologous genes into database

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Hi, we don't really support this kind of thing, so any solution will be rather ad hoc. The easiest thing to do would be, as you suggested, creating the new genes or proteins without a chromosomal location. As you discovered, this would leave them them without a proper sequence, except for whatever you put in the comment field. Another alternative would be to create one or more artificial chromosomes or contigs (you could either combine all your heterologous genes into a single artificial chromosome, or generate a different contig for each one, or anything in between). That would let you provide one or more sequence files that contained all the sequences you want to include. The map positions would of course be bogus. This (a single artificial chromosome) is actually the solution we employ for our pan-genome databases, for all genes that don't have orthologs in the primary strain. It is admittedly imperfect, and takes some work to assemble and incorporate, but I don't think we have a better solution at this time.