Which Blast database is needed with hole filler?
Hello,
I was trying to try out the hole-filler capabilities of pathologic (v19). I've done through the documentation and articles. Blast+ is installed and on my path and I have .ncbirc set up. But one basic question eludes me: what database(s) should I install? The genome and/or transcriptome and/or proteome of the organism I'm analyzing? Or the files from the most closely related organism from the curated collection?
In my first runs, when I've tried to configure the blast databases from the pathway-tools menu, I see messages on my terminal window:
Warning: Protein
#<G56-10049-MONOMER instance frame in...
The sequence length of this protein could not be computed.
This protein will be ignored.
I take this to mean that it was expecting a protein database but not finding one. (there is a db for this in my blast data directory.)
Thanks Joe Carlson